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1994-10-25
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Document 3087
DOCN M94A3087
TI Correlation between apoptosis and lack of CD28 in HIV+ pts.
DT 9412
AU Castelli F; Prati E; Brugnoni D; Malacarne F; Signorini L; Tebaldi A;
Caligaris S; Cattaneo R; Carosi G; Clinic Infectious Diseases, Dpt
Clinical Immunology, Univ.; Brescia, Italy.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):157 (abstract no. PB0056). Unique
Identifier : AIDSLINE ICA10/94369488
AB OBJECTIVES: a) to identify the possible correlation between clinical
features of HIV infection and spontaneous lymphocytes apoptosis level
(SA), b) to verify if abnormal expression of molecules involved in T
cell costimulation is a feature is a feature of cells undergoing AS.
METHODS: nuclei of non-stimulated peripheral blood lymphocytes (PBL)
cultured for 72 h have been stained by propidium iodide and SA level
measured by cytofluorimetric assay. RESULTS: 96 HIV+ pts and 69 HIV-
healthy subjects have been prospectively studied. We have observed that
the rate of apoptotic cells (1) is significantly higher in HIV+ pts than
in healthy controls (mean + DS: 13.7 + 11.8% vs 3.7 + 1.5% p < 0.001),
(2) it is higher in AIDS pts than in other HIV+ pts (19.9 + 13.6% vs 7.5
+ 4.3%, p < 0.001), (3) it is weakly. albeit significantly. inversely
correlated with both CD4+ (r = 0.44. p < 0.001) and CD8+ (r = -0.35. p <
0.001) counts, (4) Long-term survivors pts (LTS) had a significantly
lower SA level than asymptomatic HIV+ pts with a shorter follow-up and a
similar number of CD4+ circulating lymphocytes (5.8 + 2.9% vs 9.9 +
4.9%. p < 0.05). In LTS. but not in other HIV+. SA levels remained
stable over time. CD28 expression is significantly lower in CD4+ and
CD8+ from HIV pts compared to controls (CD4+ p < 0.001: CD8+ p < 0.001).
DISCUSSION AND CONCLUSION: SA levels may correlate with impaired
inter-cellular signal transduction (low CD28) and may represent the
final result of incomplete HIV mediated activation followed by
reactivation. SA levels may have prognostic significance, even if
further studies are needed.
DE Antigens, CD28/*ANALYSIS Apoptosis/*PHYSIOLOGY CD4-CD8 Ratio Flow
Cytometry Human HIV Seronegativity/IMMUNOLOGY HIV
Seropositivity/*IMMUNOLOGY/MORTALITY Lymphocyte
Transformation/IMMUNOLOGY Prospective Studies
T-Lymphocytes/*IMMUNOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).